Salut,
pour ceux que ça intéresse, c'est pas encore paru, mais presque....
Séb
in press in Molecular Ecology (special issue sometime in 2004)
Using Nuclear Haplotypes with
Microsatellites to Study Gene Flow
Between Recently Separated Cichlid Species
JODY HEY*, YONG-JIN WON*, ARJUN SIVASUNDAR*, RASMUS NIELSEN? and
JEFFREY A. MARKERT?
*Department of Genetics, Rutgers the State University of New Jersey, Piscataway, NJ 08854
USA, ?Department of Biological Statistics and Computational Biology, Cornell University,
Ithaca, NY 14853-780, USA, ? Department of Biological Sciences, University of Cincinnati,
Cincinnati, OH 45221, USA
KEYWORDS: Speciation, Microsatellites, STR, Markov chain Monte Carlo,
Cichlid, gene flow, phylogeography, sympatric speciation
Correspondence: J. Hey E-mail
hey@biology.rutgers.edu
Abstract
When populations or species have recently separated they often share genetic
variation. However it can be difficult to determine whether shared polymorphisms are the result of gene flow, or to the persistence of variation in both populations since the time of common ancestry, or to both. We have developed an empirical protocol for using loci that include unique nuclear DNA sequence haplotypes together with linked microsatellites or short tandem repeats (STRs). These ?HapSTRs? offer the potentially high resolution associated with the high mutation rate of STRs, together with the advantages of low homoplasy of unique sequence DNA. We also describe a new procedure for estimating the likelihood of HapSTR data under an Isolation with Migration model. An example using 2 Cichlid fishes from Lake Malawi is described. The analysis suggests that the species have been exchanging genes since the time they began to diverge.